Journal: Cancer cell

Article Title: CRL4B catalyzes H2AK119 monoubiquitination and coordinates with PRC2 to promote tumorigenesis.

doi: 10.1016/j.ccr.2012.10.024

Figure Lengend Snippet: Figure 7. CUL4B Is a Potential Cancer Biomarker (A) Immunohistochemical staining of CUL4B in normal esophageal tissue and esophageal carcinomas (histological grades I, II, and III). (B) The positively stained nuclei (in percentages) in grouped samples were analyzed by two-tailed unpaired t test (*p < 0.05; **p < 0.01; ***p < 0.001). (C) The expression of CUL4B mRNA and protein is upregulated in esophageal carcinomas. Total RNAs and proteins in paired samples of esophageal carcinomas versus adjacent normal esophageal tissues were extracted, and the expression of CUL4B was measured by qPCR and western blotting. mRNA levels were

Article Snippet: Since EZH2 has also been reported to be overexpressed in various cancers (Simon and Lange, 2008), we also determined the expression profile of CUL4B and EZH2 using the human cancer survey qPCR gene expression panel (Origene) in tumors originated from breast, colon, kidney, lung, ovary, and thyroid (Figure 7F).

Techniques: Biomarker Discovery, Immunohistochemical staining, Staining, Two Tailed Test, Expressing, Western Blot